Journal:

Article Title: Myeloid Cell Function in MRP-14 (S100A9) Null Mice

doi: 10.1128/MCB.23.7.2564-2576.2003

Figure Lengend Snippet: MRP-14 protein is absent in MRP-14−/− monocytes and neutrophils. (A) Myeloid cells were identified by flow cytometry by staining MRP-14+/+ and MRP-14−/− bone marrow cells with MAb 7/4. (B) The MAb 7/4-positive myeloid cells (R1) from MRP-14+/+ and MRP-14−/− mice were intracellularly stained with either MAb 2B10, specific for MRP-14 (shaded histogram), or an IgG2a isotype control MAb (open histogram). (C) In MRP-14+/+ mice, two different MRP-14-positive populations (R2 and R3) can be seen that are absent in MRP-14−/− myeloid cells. These populations correspond to monocytes (R2) and neutrophils (R3), based on their differential staining with MAb Gr-1. Open histogram represents the Gr-1-negative, 7/4-negative cells. Inset numbers represent the geometric mean fluorescence of three Gr-1-positive populations.

Article Snippet: MRP-14 was detected with MAb 2B10 (2 μg/ml) and compared to control IgG2a, followed by goat anti-rat immunoglobulin-horseradish peroxidase conjugate (1:500, Southern Biotechnology).

Techniques: Flow Cytometry, Staining, Fluorescence

Journal: Filaria Journal

Article Title: Antibody responses to Brugia malayi antigens induced by DNA vaccination

doi: 10.1186/1475-2883-3-1

Figure Lengend Snippet: This figure shows serum IgG isotype antibody responses in mice vaccinated with BM5 by intramuscular (IM) N = 7 and Gene Gun (GG), N = 8 immunization. Antigen-specific ELISAs were performed with sera collected 8 wk after the first immunization. A : IgG1 and IgG2a results shown are mean OD + SE for IM and GG mice; B : IgG1 to IgG2a ratios are shown for mice immunized with BM5 by IM and GG routes. Values shown are mean PD +SE. The ratios in the two groups were significantly different (p < 0.05).

Article Snippet: Bound antibody was detected after incubation with peroxidase-conjugated goat anti-mouse IgG, IgG1, or IgG2a antibodies (Southern Biotechnology Associates, Birmingham, AL) for 1 hr at 37°C.

Techniques:

Journal: Filaria Journal

Article Title: Antibody responses to Brugia malayi antigens induced by DNA vaccination

doi: 10.1186/1475-2883-3-1

Figure Lengend Snippet: This figure shows serum IgG isotype antibody responses in mice vaccinated with BMIF by IM (N = 12) and GG (N = 9). Antigen-specific ELISAs were performed with sera collected 8 wk after immunization. A: IgG1 and IgG2a antibodies shown are mean OD + SE; B: IgG1 to IgG2a ratios are shown for mice immunized with BMIF by IM and GG routes. Values shown are means + SE. The ratios were not significantly different.

Article Snippet: Bound antibody was detected after incubation with peroxidase-conjugated goat anti-mouse IgG, IgG1, or IgG2a antibodies (Southern Biotechnology Associates, Birmingham, AL) for 1 hr at 37°C.

Techniques:

Journal: Filaria Journal

Article Title: Antibody responses to Brugia malayi antigens induced by DNA vaccination

doi: 10.1186/1475-2883-3-1

Figure Lengend Snippet: This figure shows serum IgG isotype antibody responses in mice vaccinated with BMHSP by IM (N = 12) and GG (N = 9) route. Antigen-specific ELISAs were performed with sera collected 8 wk after immunization. A: IgG1 and IgG2a results shown are mean OD +SE; B: IgG1 to IgG2a ratios are shown for mice immunized with BMHSP by IM and GG route. Values shown are means + SE. The ratios were not significantly different.

Article Snippet: Bound antibody was detected after incubation with peroxidase-conjugated goat anti-mouse IgG, IgG1, or IgG2a antibodies (Southern Biotechnology Associates, Birmingham, AL) for 1 hr at 37°C.

Techniques:

Journal: Filaria Journal

Article Title: Antibody responses to Brugia malayi antigens induced by DNA vaccination

doi: 10.1186/1475-2883-3-1

Figure Lengend Snippet: This figure shows serum IgG isotype antibody responses in mice vaccinated with BM14 by IM (N = 12) and GG (N = 9). Antigen-specific ELISAs were performed with sera collected 8 wk after immunization. A: IgG, IgG1 and IgG2a antibody results shown are mean OD + SE; B: IgG1 to IgG2a antibody ratios are shown for mice immunized with BMHSP by IM and GG routes. Values shown are means + SE. The ratios were not significantly different.

Article Snippet: Bound antibody was detected after incubation with peroxidase-conjugated goat anti-mouse IgG, IgG1, or IgG2a antibodies (Southern Biotechnology Associates, Birmingham, AL) for 1 hr at 37°C.

Techniques:

Journal: Filaria Journal

Article Title: Antibody responses to Brugia malayi antigens induced by DNA vaccination

doi: 10.1186/1475-2883-3-1

Figure Lengend Snippet: Time course of total IgG antibodies to individual recombinant B. malayi antigens after polyvalent and monovalent DNA vaccination by IM injection. ELISAs were performed with sera collected at different times after immunization. Results shown are mean OD + SE. A: IgG antibody responses to individual antigens after polyvalent DNA vaccination (5 mice). B: IgG antibody responses to individual antigens after monovalent DNA vaccination (7 mice for BM5, 12 mice for each of the other antigens).

Article Snippet: Bound antibody was detected after incubation with peroxidase-conjugated goat anti-mouse IgG, IgG1, or IgG2a antibodies (Southern Biotechnology Associates, Birmingham, AL) for 1 hr at 37°C.

Techniques: Recombinant, Injection